New constitutive latex osmotin-like proteins lacking antifungal activity.
Identifieur interne : 000133 ( Main/Exploration ); précédent : 000132; suivant : 000134New constitutive latex osmotin-like proteins lacking antifungal activity.
Auteurs : Cleverson D T. Freitas [Brésil] ; Maria Z R. Silva [Brésil] ; Frederico Bruno-Moreno [Brésil] ; Ana C O. Monteiro-Moreira [Brésil] ; Renato A. Moreira [Brésil] ; Márcio V. Ramos [Brésil]Source :
- Plant physiology and biochemistry : PPB [ 1873-2690 ] ; 2015.
Descripteurs français
- KwdFr :
- Antifongiques (composition chimique), Antifongiques (pharmacologie), Chromatographie d'affinité (méthodes), Données de séquences moléculaires (MeSH), Latex (composition chimique), Protéines végétales (composition chimique), Protéines végétales (pharmacologie), Similitude de séquences d'acides aminés (MeSH), Séquence d'acides aminés (MeSH).
- MESH :
- composition chimique : Antifongiques, Latex, Protéines végétales.
- méthodes : Chromatographie d'affinité.
- pharmacologie : Antifongiques, Protéines végétales.
- Données de séquences moléculaires, Similitude de séquences d'acides aminés, Séquence d'acides aminés.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Antifungal Agents, Latex, Plant Proteins.
- chemical , pharmacology : Antifungal Agents, Plant Proteins.
- methods : Chromatography, Affinity.
- Amino Acid Sequence, Molecular Sequence Data, Sequence Homology, Amino Acid.
Abstract
Proteins that share similar primary sequences to the protein originally described in salt-stressed tobacco cells have been named osmotins. So far, only two osmotin-like proteins were purified and characterized of latex fluids. Osmotin from Carica papaya latex is an inducible protein lacking antifungal activity, whereas the Calotropis procera latex osmotin is a constitutive antifungal protein. To get additional insights into this subject, we investigated osmotins in latex fluids of five species. Two potential osmotin-like proteins in Cryptostegia grandiflora and Plumeria rubra latex were detected by immunological cross-reactivity with polyclonal antibodies produced against the C. procera latex osmotin (CpOsm) by ELISA, Dot Blot and Western Blot assays. Osmotin-like proteins were not detected in the latex of Thevetia peruviana, Himatanthus drasticus and healthy Carica papaya fruits. Later, the two new osmotin-like proteins were purified through immunoaffinity chromatography with anti-CpOsm immobilized antibodies. Worth noting the chromatographic efficiency allowed for the purification of the osmotin-like protein belonging to H. drasticus latex, which was not detectable by immunoassays. The identification of the purified proteins was confirmed after MS/MS analyses of their tryptic digests. It is concluded that the constitutive osmotin-like proteins reported here share structural similarities to CpOsm. However, unlike CpOsm, they did not exhibit antifungal activity against Fusarium solani and Colletotrichum gloeosporioides. These results suggest that osmotins of different latex sources may be involved in distinct physiological or defensive events.
DOI: 10.1016/j.plaphy.2015.07.012
PubMed: 26231325
Affiliations:
Links toward previous steps (curation, corpus...)
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Moreira</name><affiliation wicri:level="2"><nlm:affiliation>Centro de Ciências da Saúde, Universidade de Fortaleza, Unifor, Fortaleza-CE, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Centro de Ciências da Saúde, Universidade de Fortaleza, Unifor, Fortaleza-CE</wicri:regionArea><placeName><region type="state">Ceará</region></placeName></affiliation></author><author><name sortKey="Ramos, Marcio V" sort="Ramos, Marcio V" uniqKey="Ramos M" first="Márcio V" last="Ramos">Márcio V. Ramos</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Bioquímica e Biologia Molecular da Universidade Federal do Ceará, Campus do Pici, Cx. Postal 6033, Fortaleza, Ceará, CEP 60451-970, Brazil. Electronic address: vramos@ufc.br.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Bioquímica e Biologia Molecular da Universidade Federal do Ceará, Campus do Pici, Cx. Postal 6033, Fortaleza, Ceará, CEP 60451-970</wicri:regionArea><wicri:noRegion>CEP 60451-970</wicri:noRegion></affiliation></author></analytic><series><title level="j">Plant physiology and biochemistry : PPB</title><idno type="eISSN">1873-2690</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Antifungal Agents (chemistry)</term><term>Antifungal Agents (pharmacology)</term><term>Chromatography, Affinity (methods)</term><term>Latex (chemistry)</term><term>Molecular Sequence Data (MeSH)</term><term>Plant Proteins (chemistry)</term><term>Plant Proteins (pharmacology)</term><term>Sequence Homology, Amino Acid (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Antifongiques (composition chimique)</term><term>Antifongiques (pharmacologie)</term><term>Chromatographie d'affinité (méthodes)</term><term>Données de séquences moléculaires (MeSH)</term><term>Latex (composition chimique)</term><term>Protéines végétales (composition chimique)</term><term>Protéines végétales (pharmacologie)</term><term>Similitude de séquences d'acides aminés (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antifungal Agents</term><term>Latex</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antifungal Agents</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Antifongiques</term><term>Latex</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromatography, Affinity</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Chromatographie d'affinité</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antifongiques</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Molecular Sequence Data</term><term>Sequence Homology, Amino Acid</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Données de séquences moléculaires</term><term>Similitude de séquences d'acides aminés</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Proteins that share similar primary sequences to the protein originally described in salt-stressed tobacco cells have been named osmotins. So far, only two osmotin-like proteins were purified and characterized of latex fluids. Osmotin from Carica papaya latex is an inducible protein lacking antifungal activity, whereas the Calotropis procera latex osmotin is a constitutive antifungal protein. To get additional insights into this subject, we investigated osmotins in latex fluids of five species. Two potential osmotin-like proteins in Cryptostegia grandiflora and Plumeria rubra latex were detected by immunological cross-reactivity with polyclonal antibodies produced against the C. procera latex osmotin (CpOsm) by ELISA, Dot Blot and Western Blot assays. Osmotin-like proteins were not detected in the latex of Thevetia peruviana, Himatanthus drasticus and healthy Carica papaya fruits. Later, the two new osmotin-like proteins were purified through immunoaffinity chromatography with anti-CpOsm immobilized antibodies. Worth noting the chromatographic efficiency allowed for the purification of the osmotin-like protein belonging to H. drasticus latex, which was not detectable by immunoassays. The identification of the purified proteins was confirmed after MS/MS analyses of their tryptic digests. It is concluded that the constitutive osmotin-like proteins reported here share structural similarities to CpOsm. However, unlike CpOsm, they did not exhibit antifungal activity against Fusarium solani and Colletotrichum gloeosporioides. These results suggest that osmotins of different latex sources may be involved in distinct physiological or defensive events. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26231325</PMID><DateCompleted><Year>2016</Year><Month>09</Month><Day>02</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-2690</ISSN><JournalIssue CitedMedium="Internet"><Volume>96</Volume><PubDate><Year>2015</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Plant physiology and biochemistry : PPB</Title><ISOAbbreviation>Plant Physiol Biochem</ISOAbbreviation></Journal><ArticleTitle>New constitutive latex osmotin-like proteins lacking antifungal activity.</ArticleTitle><Pagination><MedlinePgn>45-52</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.plaphy.2015.07.012</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0981-9428(15)30058-9</ELocationID><Abstract><AbstractText>Proteins that share similar primary sequences to the protein originally described in salt-stressed tobacco cells have been named osmotins. So far, only two osmotin-like proteins were purified and characterized of latex fluids. Osmotin from Carica papaya latex is an inducible protein lacking antifungal activity, whereas the Calotropis procera latex osmotin is a constitutive antifungal protein. To get additional insights into this subject, we investigated osmotins in latex fluids of five species. Two potential osmotin-like proteins in Cryptostegia grandiflora and Plumeria rubra latex were detected by immunological cross-reactivity with polyclonal antibodies produced against the C. procera latex osmotin (CpOsm) by ELISA, Dot Blot and Western Blot assays. Osmotin-like proteins were not detected in the latex of Thevetia peruviana, Himatanthus drasticus and healthy Carica papaya fruits. Later, the two new osmotin-like proteins were purified through immunoaffinity chromatography with anti-CpOsm immobilized antibodies. Worth noting the chromatographic efficiency allowed for the purification of the osmotin-like protein belonging to H. drasticus latex, which was not detectable by immunoassays. The identification of the purified proteins was confirmed after MS/MS analyses of their tryptic digests. It is concluded that the constitutive osmotin-like proteins reported here share structural similarities to CpOsm. However, unlike CpOsm, they did not exhibit antifungal activity against Fusarium solani and Colletotrichum gloeosporioides. These results suggest that osmotins of different latex sources may be involved in distinct physiological or defensive events. </AbstractText><CopyrightInformation>Copyright © 2015 Elsevier Masson SAS. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Freitas</LastName><ForeName>Cleverson D T</ForeName><Initials>CD</Initials><AffiliationInfo><Affiliation>Departamento de Bioquímica e Biologia Molecular da Universidade Federal do Ceará, Campus do Pici, Cx. Postal 6033, Fortaleza, Ceará, CEP 60451-970, Brazil. Electronic address: cleversondiniz@hotmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Silva</LastName><ForeName>Maria Z R</ForeName><Initials>MZ</Initials><AffiliationInfo><Affiliation>Departamento de Bioquímica e Biologia Molecular da Universidade Federal do Ceará, Campus do Pici, Cx. Postal 6033, Fortaleza, Ceará, CEP 60451-970, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bruno-Moreno</LastName><ForeName>Frederico</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Centro de Ciências da Saúde, Universidade de Fortaleza, Unifor, Fortaleza-CE, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Monteiro-Moreira</LastName><ForeName>Ana C O</ForeName><Initials>AC</Initials><AffiliationInfo><Affiliation>Centro de Ciências da Saúde, Universidade de Fortaleza, Unifor, Fortaleza-CE, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Moreira</LastName><ForeName>Renato A</ForeName><Initials>RA</Initials><AffiliationInfo><Affiliation>Centro de Ciências da Saúde, Universidade de Fortaleza, Unifor, Fortaleza-CE, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ramos</LastName><ForeName>Márcio V</ForeName><Initials>MV</Initials><AffiliationInfo><Affiliation>Departamento de Bioquímica e Biologia Molecular da Universidade Federal do Ceará, Campus do Pici, Cx. Postal 6033, Fortaleza, Ceará, CEP 60451-970, Brazil. Electronic address: vramos@ufc.br.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>07</Month><Day>17</Day></ArticleDate></Article><MedlineJournalInfo><Country>France</Country><MedlineTA>Plant Physiol Biochem</MedlineTA><NlmUniqueID>9882449</NlmUniqueID><ISSNLinking>0981-9428</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000935">Antifungal Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007840">Latex</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000935" MajorTopicYN="N">Antifungal Agents</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002846" MajorTopicYN="N">Chromatography, Affinity</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007840" MajorTopicYN="N">Latex</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017386" MajorTopicYN="N">Sequence Homology, Amino Acid</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Immunoaffinity chromatography</Keyword><Keyword MajorTopicYN="N">Mass spectrometry</Keyword><Keyword MajorTopicYN="N">Phytopathogens</Keyword><Keyword MajorTopicYN="N">Thaumatin</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>02</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>05</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>07</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>8</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>8</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>9</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26231325</ArticleId><ArticleId IdType="pii">S0981-9428(15)30058-9</ArticleId><ArticleId IdType="doi">10.1016/j.plaphy.2015.07.012</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Brésil</li></country><region><li>Ceará</li></region></list><tree><country name="Brésil"><noRegion><name sortKey="Freitas, Cleverson D T" sort="Freitas, Cleverson D T" uniqKey="Freitas C" first="Cleverson D T" last="Freitas">Cleverson D T. Freitas</name></noRegion><name sortKey="Bruno Moreno, Frederico" sort="Bruno Moreno, Frederico" uniqKey="Bruno Moreno F" first="Frederico" last="Bruno-Moreno">Frederico Bruno-Moreno</name><name sortKey="Monteiro Moreira, Ana C O" sort="Monteiro Moreira, Ana C O" uniqKey="Monteiro Moreira A" first="Ana C O" last="Monteiro-Moreira">Ana C O. Monteiro-Moreira</name><name sortKey="Moreira, Renato A" sort="Moreira, Renato A" uniqKey="Moreira R" first="Renato A" last="Moreira">Renato A. Moreira</name><name sortKey="Ramos, Marcio V" sort="Ramos, Marcio V" uniqKey="Ramos M" first="Márcio V" last="Ramos">Márcio V. Ramos</name><name sortKey="Silva, Maria Z R" sort="Silva, Maria Z R" uniqKey="Silva M" first="Maria Z R" last="Silva">Maria Z R. Silva</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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